Researchers from CSIC and Immunostep have released a test that can detect three antibodies against coronavirus proteins in the blood, including antibodies that have not yet been used for diagnosis. In this way, you can meet people who have been in contact with the virus and have been immunized.
This Serology COVID-19 antibody test by researchers from Advanced Council for Scientific Research (CSIC) Cooperation with hospitals in Madrid The Princess and La PazIt has close to 100% reliability and is already on the market. The test can detect three types of antibodies and let us know about people who have been in contact with the coronavirus and have been immunized.
The test is shown in the form of an ELISA kit with all reagents to prove the existence Antibodies in the blood Patient.It was carried out in the laboratory and provided some results a couple of hours.The test has been patented by the vice chairman of CSIC Knowledge Transfer and has been licensed to the biotech company Immune step, It is produced in Salamanca.
He explained: “The main novelty of this test is that it is based on a viral protein, which has not been studied for diagnosis so far.” Mar Valés,researcher CSIC National Biotechnology Center (CNB-CSIC), Who is the co-leader of the investigation, he added: “We found that Protease, Is a protein produced by the virus during the infection process, which can act as an antigen. Patients develop antibodies that can be detected in blood samples.”
The design of the protease is led by researchers Hugh RayburnCNB-CSIC. After purification, the protein can be used to capture antibodies against coronavirus contained in the serum (blood) of individuals who have passed the disease and have developed immunity. Valés explained that the kit makes it possible to measure this reaction by changing the color.
Researchers conducted a trial, which was published in Journal of Immunology, Indicating that the test is combined with the detection of other commonly used coronavirus antigens, and was produced on CNB-CSIC by the Reyburn and José María Casasnovas team, so that all individual coronaviruses that are immune to HCV can be identified.
“The test is very sensitive,” he said. “The combination of neoantigens and immunoglobulins IgG and IgA can actually detect antibodies from the onset of symptoms.” Ricardo Jara, CEO of Immunostep. He added: “Given its high sensitivity, we think it will be a very powerful tool in this vaccination phase we are starting now.”
He said: “This test can more extensively study the immune response of antibodies against the virus.” Eduardo LopezHead of the Department of Immunology, La Paz University Hospital, Madrid. He added: “It is possible to see more antibody responses to most proteins than is usually included in most tests, including the discovery of the CSIC group, which is this protein (protease), which produces a response to the antibody. “This is why we think the test is more versatile than the existing tests. “
Qualitative and quantitative testing
Virus diagnostic tests are divided into two main types: tests that perform direct or indirect detection.When testing Polymerase chain reaction Detect the genetic material of the virus and Antigen test Detect the protein on the surface of the virus (direct detection), Antibody test Detection of proteins produced by the immune system after coronavirus infection (indirect detection). The results provide important information about the number of people immunized against covid-19.
This new test not only provides a qualitative diagnosis (indicating the presence of three antibodies), but also a quantitative diagnosis, that is, the concentration of these antibodies can be understood.
“It can identify three types of antibodies, called immunoglobulins: Immunoglobulin, Or immunoglobulin M, is produced first after infection, usually five or six days after the onset of symptoms.It means that the body is beginning to respond to the disease and the infection may be occurring (the patient is still contagious); this ImmunoglobulinOr immunoglobulin G is produced at a later time, lasting several weeks or months, so it helps to understand whether a person has the disease in the past; and age, Or immunoglobulin A that appears when a virus is found in the mucous membrane”, the researchers said Jose Miguel Rodriguez FredHe is the co-director of CNB-CSIC.
Help during vaccination
According to its creator, it is a very useful tool that can be used to identify people who are immune to the coronavirus. Therefore, during the immunization process Population vaccination. Through this test, it is possible to distinguish who has responded to the vaccine, who has responded to the vaccine, and has responded to the intact virus.