Researchers at the Institute of Materials Science in Barcelona (CSIC) have developed organic nanoparticles with fluorescent molecules that emit light more than 100 times stronger than similar molecules such as quantum dots. This advancement can help obtain higher-quality biological images and can be applied to medical diagnostic equipment.
In the past few decades Super Resolution Fluorescence Microscope It allows us to see the contents of the cell and reach the nanometer level with higher and higher resolution.
in this regard Fluorescent probeOnce the molecule is excited, it emits light at a specific wavelength and must meet a series of requirements, such as high luminosity or brightness, complete biocompatibility, high photostability and high dispersion in physiological media.
Group researcher Nanomole of Barcelona Institute of Materials Science (ICMAB-CSIC)Together with colleagues from the New Jersey Institute of Technology (USA) and the University of Parma (Italy), developed this type of new fluorescent probe: Fluorescent organic nanoparticles (FON, With English initials).
They are based on the so-called Quadruple body, Are nanovesicles produced by the same group through sustainable technology, which contain fluorophores or fluorescent molecules, especially two types of anthocyanins.
The average diameter of the nanoparticles is 120 nm Over time and once exposed to high-power laser irradiation, they all show good biocompatibility and high stability, thus forming a new type of nanomaterial for bioimaging.
“The brightness obtained is especially important because the brightness of these new fluorescent nanovesicles is higher than that of other commercial fluorescent nanovesicles (such as quantum dots or Quantum dots, So you can get high-quality images” Judit Morla-Folch, ICMAB researcher, the first author of the study, has been published in the journal ACS application alma mater interface.
Förster to reduce image noise
In addition, these fluorescent nanoparticles have another unique feature: they have experienced the so-called Foster resonance energy transfer, Usually abbreviated as FRET, is its English abbreviation.
This phenomenon helps to improve image acquisition, because self-absorption and background noise during biological image acquisition are greatly reduced. It also allows monitoring of the integrity of the nanoparticles, which is a great advantage for biomedical applications that need to know when nanovesicles remain intact or disintegrate.
According to their creators, these new FONs are very promising Biological imaging platform And for Medical diagnostic kit.
Judit Morla-Folch, Guillem Vargas-Nadal, Tinghan Zhao, Cristina Sissa, Antonio Ardizzone, Siarhei Kurhuzenkau, Mariana Köber, Mehrun Uddin, Anna Painelli, Jaume Veciana, Kevin D. Belfield and Nora Ventosa. “Showing FRET as a dye quartet for bioimaging nanoprobes”. ACS application alma mater interface, 2020.
The research results are the result of the TECNIOspring PLUS project co-funded by ACCIÓ and the European Commission. ICMAB-CSIC Be part of the network Siber-BBN And ICTS Nanbiosis U6 And TECNIO technology transfer network action–Government of Catalonia.