Employees of public enterprises in the transport, mining, teachers and students sectors have been on guard for months, according to the opposition, which the power denies.
Tihanovskaia took part in a march in Copenhagen with dozens of supporters three days ago in the Danish capital.
She calls on the strongest man in Belarus to end the violence and release political prisoners. Her husband, who ran in the August 9th election, is in custody.
However, Alexander Lukashenko, 66, who has been in power since 1994, is clinging to the chair following the allegedly fraudulent election, despite international pressure.
Tikhanovskaya announced on Monday the start of a strike calling for Lukashenko to step down.
“Since this morning, employees of some public enterprises and factories, in the transport sector, miners, teachers and students have gone on strike,” she announced in a message posted on Telegram.
She urges her compatriots to show that “no one will work for the regime” of Alexander Lukashenko.
“The strike in public enterprises is a lever of economic pressure. And the one in the private sector is a sign of solidarity for everyone, just as important “, she underlines.
Opposition candidate Svetlana Tihanovskaia, 38, who ran in Lithuania after the election, gave an ultimatum to Alexander Lukashenko to withdraw from power by Sunday, threatening otherwise. general strike.
Opposition media outlets posted video footage on social media on Monday showing dozens of young people gathered in front of universities or in sit-ins.
The independent publication Tut.by has published images showing workers who have stopped working and solidarity actions in at least four large public factories.
“It is difficult to know how far people will go, due to high pressure from the authorities,” Aleksandr Yarushuk, the leader of the Belarusian Confederation of Democratic Trade Unions, told AFP, adding that he had not called for a strike.
A spokeswoman for the prime minister announced on Facebook that businesses in the country are operating normally.
“Some of our employees went on strike,” said Natalia Bezrukova, 54, a striker at a public construction company who was interviewed by AFP in central Minsk.
Along with her, participating in a human chain of about 50 people, Elena Velisko, 43, an entrepreneur, decided, in turn, not to work. “I am prepared for many things to be given the right to vote,” she said.
The contestation is subject to constant pressure from the elections by the authorities.
More than 100,000 people attended a new rally in Minsk on Sunday.
A total of 523 people were arrested on Sunday in the wake of protests across the country, according to the Belarusian Interior Ministry.